Cd68 Rat Monoclonal Antibody [Clone ID: FA-11]

Specifications

Product Data
Clone Name	FA-11
Applications	FC, IF, IHC, IP, WB
Recommend Dilution	Western Blot: Non-reducing conditions recommended. Immunoprecipiation. Immunofluorescence. Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 106 cells in 100 µl (Membrane permeabilization is required). Clone FA-11 can be used in Flow Cytometry to detect intracellular CD68, following permeabilization, and can detect surface macrosialin at low levels in resident Mouse peritoneal macrophages which can be enhanced with thioglycollate stimulation. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections. Rat anti Mouse CD68 antibody, Clone FA-11, has been used in many mouse models for the identification of CD68 in immunohistochemical studies, using both frozen and paraffin-embedded tissues, See Masaki, T. et al. and Devey, L. et al. as examples. -This product may require antigen retrieval using heat treatment prior to staining of paraffin sections. Either sodium citrate buffer or Tris/EDTA buffer may be used for this purpose. See Martin-Manso for details. -Staining has also been achieved without antigen retrieval, See Lu et al. for details.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	Purified Concanavalin A acceptor glycoprotein from P815 cell line.
Specificity	Clone FA-11 recognizes mouse macrosialin, a heavily glycosylated transmembrane protein and murine homolog of human CD68, which is classified as a unique scavenger receptor (ScR) family member, due to the presence of a lysosome associated membrane protein (LAMP)-like domain.
Formulation	PBS State: Purified State: Liquid purfied IgG fraction from Tissue Culture Supernatant Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	CD68 antigen
Database Link	Entrez Gene 12514 Mouse UniProt ID P31996
Background	CD68 is considered a pan macrophage marker, predominantly expressed on the intracellular lysosomes of tissue macrophages/monocytes, including Kupffer cells, microglia, histiocytes and osteoclasts, and is expressed to a lesser extent by dendritic cells and peripheral blood granulocytes. CD68 is expressed by many tumor types including some B cell lymphomas, blastic NK lymphomas, melanomas, granulocytic (myeloid) sarcomas, hairy cell leukemias, and renal, urinary and pancreatic tumors, and can be used in cancer studies to demonstrate the presence/localization of macrophages.
Synonyms	Gp110, Macrosialin, Macrophage marker
Reference Data

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