MCM5 Mouse Monoclonal Antibody [Clone ID: CRCT5.1]

Specifications

Product Data
Clone Name	CRCT5.1
Applications	IHC, WB
Recommend Dilution	Western Blot. Immunohistochemistry on Paraffin Sections (1/50-1/1000): This product requires antigen retrieval using heat treatment prior to staining of paraffin sections; Sodium citrate buffer pH6.0 is recommended for this purpose. Recommended Positive Control: Tonsil / Cervical Carcinoma.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Bacterially expressed human Mcm5. Spleen cells from immunised CBI/CFI mice were fused with cells of the mouse SP2 myeloma cell line.
Specificity	This antibody recognises Mcm-5 (minichromosome maintenance protein 5).
Formulation	PBS, pH7.4 containing 0.09% Sodium Azide as preservative. State: Purified State: Liquid purified IgG fraction.
Concentration	lot specific
Purification	Affinity chromatography on Protein G.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	minichromosome maintenance complex component 5
Database Link	Entrez Gene 4174 Human UniProt ID P33992
Background	Mcm-5 is a protein with an important role in the control of DNA replication. It has been reported that immunocytochemical assessment of Mcm5 expression may be of value in improving the accuracy of cervical smear testing for the detection of malignancy.
Synonyms	CDC46 homolog, P1-CDC46, CDC46
Reference Data

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