Myeloperoxidase (MPO) Mouse Monoclonal Antibody [Clone ID: 2C7]

Specifications

Product Data
Clone Name	2C7
Applications	FC
Recommend Dilution	Flow Cytometry: Use 10 µl of neat antibody to label 10e6 cells in 100 µl (Membrane permeabilisation is required).
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human Myeloperoxiase. Spleen cells from immunised mice were fused with cells of the mouse X63 AG8-653 myeloma cell line.
Specificity	This antibody recognizes Myeloperoxidase (MPO). It may be used for the Flow Cytometric detection of Myeloid cells following membrane permeablisation. Negative Species: Rat.
Formulation	PBS Label: PE State: Lyophilized purified IgG fraction Stabilizer: 1% BSA, 5% Sucrose Preservative: 0.09% Sodium Azide Label: R. Phycoerythrin (RPE)
Reconstitution Method	Restore with 1 ml distilled water
Purification	Affinity Chromatography on Protein G
Conjugation	PE
Storage Condition	Store undiluted Prior to and following reconstitution at 2-8°C. DO NOT FREEZE! This antibody is photosensitive and should be protected from light.
Gene Name	myeloperoxidase
Database Link	Entrez Gene 4353 Human UniProt ID P05164
Background	MPO is an important component of azurophilic granules in neutrophils. Myeloperoxidase is a hemoprotein that is abundantly expressed in neutrophils and secreted during their activation. Native Myeloperoxidase is represented as a covalently bound tetrameric complex of two glycosylated alpha chains (MW 59 - 64 kDa) and two unglycosylated beta chains (MW 14 kDa) with total MW 150 kDa and theoretical pI 9.2. Traditionally Myeloperoxidase was considered as a main target of anti-neutrophil cytoplasm antibodies (ANCA), the serological markers for certain systemic vasculities e.g. periarteriitis nodosa, microscopic polyarteriitis and pulmonary eosinophilic granulomatosis (Churg-Strauss syndrome). Low to moderate anti-Myeloperoxidase autoantibody levels are also reported in rheumatoid arthritis. Recently it was shown that Myeloperoxidase participates in the initiation and progression of cardiovascular disease. It possesses potent proinflammatory properties and may contribute directly to tissue injury. Now Myeloperoxidase is under consideration as one of the most promising cardiac markers.
Synonyms	MPO
Reference Data

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