PARP1 Mouse Monoclonal Antibody [Clone ID: A6.4.12]

Specifications

Product Data
Clone Name	A6.4.12
Applications	ELISA, IHC, IP, WB
Recommend Dilution	ELISA. Immunoprecipitation. Western Blot: 1/1000-1/5000. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections; Sodium citrate buffer pH 6.0 is recommended for this purpose.
Reactivity	Drosophila, Hamster, Human, Mouse, Rat, Xenopus
Host	Mouse
Clonality	Monoclonal
Immunogen	Human PARP. Spleen cells from immunised BALB/c mice were fused with cells of mouse NSO myeloma cell line.
Specificity	This antibody recognises poly (ADP-ribose) polymerase (PARP).
Formulation	State: Aff - Purified State: Liquid purified IgG containing 0.09% Sodium Azide as preservative.
Concentration	lot specific
Purification	Affinity Chromatography on Protein G.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	poly(ADP-ribose) polymerase 1
Database Link	Entrez Gene 142 Human UniProt ID P09874
Background	PARP (Poly (ADP-ribose) polymerase) is a 113 kDa nuclear protein which can exist as a homo- or hetero-dimer, and is strongly activated by DNA strand breaks. This protein acts as a molecular ''nick sensor'' and functions in base excision repair, poly(ADPribosyl)ation of acceptor proteins involved in chromatin architecture and DNA metabolism, and participates in protein modification to enhance or repress transcription. PARP also plays a role in other cellular processes, including cell proliferation and differentiation. PARP is ribosylated by PARP2, and during apoptosis, ICE family members, such as caspase 3 and 7, cleave PARP to yield an 85 kDa and a 25 kDa fragment. PARP cleavage is considered to be one of the classical characteristics of apoptosis. PARP interacts with proteins in the base excision repair complex containing at least XRCC1, PARP2, POLB and LIG3. In addition PARP forms heterodimers with PARP2, and interacts with PARP3.
Synonyms	PARP-1, ADPRT, ADPRT 1, PPOL
Reference Data

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