Actin (F-Actin) Mouse Monoclonal Antibody [Clone ID: NH3]

Specifications

Product Data
Clone Name	NH3
Applications	E, FC, IF, IHC, WB
Recommend Dilution	ELISA: 1/10. Western Blotting: 1/100-1/500. Flow Cytometry: 1/10 Immunofluorescence. Immunohistochemistry on Frozen Sections.
Reactivity	Human, Mouse, Rabbit, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Human monocytes and U937 cell line. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Specificity	This antibody recognizes Filamentous Actin (F-Actin). The antibody binds to the N-terminal region of actin, but not to the extreme N-terminal 40 amino acids. In tissue sections the antibody stains the cytoplasm of macrophages strongly, and gives granular, localised nuclear staining of all cell types. Clone NH3 is reported to recognize Actin in the filamentous form with the epitope likely to be located between residues 120 and 226 of the molecule. Clone NH3 is also described to show reactivity with a 43 kDa polypeptide using cell lines U937 and HL-60 by SDS/PAGE and Immunoblotting.
Formulation	PBS, pH 7.4 State: Purified State: Liquid purified IgM fraction prepared from tissue culture supernatant. Preservative: 0.09% Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. G-Actin (globular actin) with bound ATP can polymerise to form F-Actin (Filamentious Actin). Actin monomers spiral ariound the axis of the filament similar to a double helix. F-Actin may also undergo a process called treadmilling, in which filament length remains constant and actin monomers add at one end and dissociated at the other.
Synonyms	Actin F type
Reference Data

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