Actin (F-Actin) Mouse Monoclonal Antibody [Clone ID: NH3]
CAT#: SM1349P
Actin (F-Actin) mouse monoclonal antibody, clone NH3, Purified
Size: 50 ug
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CNY 7,912.00
货期*
4周
规格
Cited in 7 publications. |
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Specifications
Product Data | |
Clone Name | NH3 |
Applications | ELISA, FC, IF, IHC, WB |
Recommend Dilution | ELISA: 1/10. Western Blotting: 1/100-1/500. Flow Cytometry: 1/10 Immunofluorescence. Immunohistochemistry on Frozen Sections. |
Reactivity | Human, Mouse, Rabbit, Rat |
Host | Mouse |
Clonality | Monoclonal |
Immunogen | Human monocytes and U937 cell line. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line. |
Specificity | This antibody recognizes Human Filamentous Actin (F-actin). The antibody binds to the N-terminal region of Actin, but not to the extreme N-terminal 40 amino acids. In tissue sections the antibody stains the cytoplasm of macrophages strongly, and gives granular, localised nuclear staining of all cell types. Clone NH3 is reported to recognize Actin in the filamentous form with the epitope likely to be located between residues 120 and 226 of the molecule. Clone NH3 is also described to show reactivity with a 43 kDa polypeptide using cell lines U937 and HL-60 by SDS/PAGE and Immunoblotting. |
Formulation | PBS State: Purified State: Liquid purified IgM fraction prepared from Tissue Culture Supernatant Preservative: 0.09% Sodium Azide |
Concentration | lot specific |
Conjugation | Unconjugated |
Storage Condition | Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
Background | Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. G-Actin (globular actin) with bound ATP can polymerise to form F-Actin (Filamentious Actin). Actin monomers spiral ariound the axis of the filament similar to a double helix. F-Actin may also undergo a process called treadmilling, in which filament length remains constant and actin monomers add at one end and dissociated at the other. |
Synonyms | Actin F type |
Reference Data |
Citations (7)
The use of this Antibodies has been cited in the following citations: |
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FICC-Seq: a method for enzyme-specified profiling of methyl-5-uridine in cellular RNA
,null,
Nucleic Acids Research
,PubMed ID 31361898
|
Effects of High-cholesterol Diet on Pravastatin Disposition in the Perfused Rat Liver.
,null,
European journal of drug metabolism and pharmacokinetics
,PubMed ID 27511381
|
Contribution of myo-inositol oxygenase in AGE:RAGE-mediated renal tubulointerstitial injury in the context of diabetic nephropathy
,null,
American Journal of Physiology - Renal Physiology
,PubMed ID 28931523
|
EPHB4, a down stream target of IFN-γ/STAT1 signal pathway, regulates endothelial activation possibly contributing to the development of preeclampsia.
,null,
American journal of reproductive immunology (New York, N.Y. : 1989)
,PubMed ID 27553867
|
Caffeine inhibits hypothalamic A1R to excite oxytocin neuron and ameliorate dietary obesity in mice
,null,
Nature Communications
,PubMed ID 28654087
|
Palbociclib induces activation of AMPK and inhibits hepatocellular carcinoma in a CDK4/6‐independent manner
,null,
Molecular Oncology
,PubMed ID 28453226
|
Hypoxic induction of receptor activity-modifying protein 2 alters regulation of pulmonary endothelin-1 by adrenomedullin: induction under normoxia versus inhibition under hypoxia.
,null,
The Journal of pharmacology and experimental therapeutics
,PubMed ID 17251392
|
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