Glucocorticoid Receptor (NR3C1) Mouse Monoclonal Antibody [Clone ID: 8E9]

Specifications

Product Data
Clone Name	8E9
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA: 1/1000. Western Blot: 1/1000. Successful use in Immunohistochemistry on frozen and paraffin sections was reported by some researchers. As this detection method has not been verified by Acris Antibodies, the application was deleted from the database. This does not necessarily exclude the use in such procedure.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	26 amino acid peptide corresponding to residues 150-176 of human GCR linked to thyroglobulin (human). Spleen cells from immunised BALB/c mice were fused with cells of the mouse Sp-2/0 Ag14 myeloma cell line.
Specificity	This antibody recognises the glucocorticoid receptor, located in the cytoplasm of cells, and associated with certain heat shock proteins. Clone 8E9 binds to an epitope between amino acids 167-176.
Formulation	PBS, pH7.2 containing 0.09% Sodium Azide and 0.1% Bovine Serum Albumin State: Purified State: Liquid purified IgG fraction.
Concentration	lot specific
Purification	Affinity chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	nuclear receptor subfamily 3 group C member 1
Database Link	Entrez Gene 2908 Human UniProt ID P04150
Background	Steroid receptors are ligand-dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Glucocorticoids are a family of steroids necessary for the regulation of energy metabolism and the immune and inflammatory responses. These compounds exert their effect through their interaction with the glucocoticoid receptor (GR) and that complex's subsequent association with DNA. All normal mammalian tissues examined to date have been shown to contain glucocorticoid receptor.
Synonyms	NR3C1, GR, GRL
Reference Data

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