CD95 (FAS) Mouse Monoclonal Antibody [Clone ID: LOB 3/17]

CAT#: SM1203PT

CD95 (FAS) mouse monoclonal antibody, clone LOB 3/17, Purified



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CNY 2,570.00


货期*
4周

规格
    • 25 ug

Product images

Specifications

Product Data
Clone Name LOB 3/17
Applications FC, IP
Recommend Dilution Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 10e6 cells in 100µl.
Immunoprecipitation: 20 µg/ml.
Reactivity Human, Monkey
Host Mouse
Clonality Monoclonal
Immunogen Fusion protein comprising extracellular domain of human Fas linked to human Fc.
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NSI myeloma cell line.
Specificity This antibody recognises the CD95 cell surface antigen.
Formulation Tris NaCl, pH 8.0 containing 0.09% Sodium Azide as preservative.
State: Purified
State: Liquid purified IgG fraction.
Concentration lot specific
Purification Affinity Chromatography on Protein A.
Conjugation Unconjugated
Storage Condition Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Gene Name Fas cell surface death receptor
Background CD95, also known as FAS or APO1, is a 36 kDa cell surface type I membrane glycoprotein with an apparent molecular weight of 44 kDa on SDS PAGE. CD95 is a member of the TNF receptor family, which includes TNFR1, TNFR2, CD27, CD30 and CD40. Binding of CD95 Ligand to CD95 or crosslinking of CD95 by anti CD95 monoclonal antibodies leads to apoptosis of CD95 expressing cells. CD95 belongs to a subgroup of family members that have a death domain (DD) which contains 70 amino acids near the carboxyl terminal region of the molecule. The binding of adaptor molecules to this DD is responsible for transmitting the death signal for apoptosis. Stimulation of CD95 results in aggregation of its DD, leading to the recruitment of FADD and caspase 8 that together with the receptor form the death inducing signaling complex (DISC). CD95/CD95L is involved in the peripheral deletion of activated mature T cells at the end of the immune response and defects in this pathway predispose to autoimmune disorders. CD95 is also involved in killing of targets such as virus infected cells or cancer cells and killing of inflammatory cells at immune privileged sites.
CD95 is expressed by activated T and B cells, NK cells and thymocytes.
Synonyms FASLG receptor, Apo-1 antigen, APT1, FAS1, TNFRSF6
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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