C5R1 (C5AR1) (N-term) Mouse Monoclonal Antibody [Clone ID: S5/1]

Specifications

Product Data
Clone Name	S5/1
Applications	FC, IHC, WB
Recommend Dilution	Flow Cytometry: Use 10µl of 1/50-1/100 diluted antibody to label 5 x 105 cells in 100 µl. Immunohistochemistry on Paraffin Sections. Western Blot. Functional Assays.
Reactivity	Bovine, Human, Rabbit
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic peptide comprising the N-terminal extracellular domain (Met1 - Asn31) of the C5a receptor (C5aR) CD88.  Spleen cells from immunised BALB/c mice were fused with cells of the X63-Ag8 myeloma cell line.
Specificity	This antibody recognizes the C5a receptor (C5aR) CD88. Clone S5/1 was raised against a synthetic peptide comprising the N-terminal extracellular domain of the C5aR (met1-Asn31) and has recently been shown to recognise the heptameric peptide (D15DKDTLD21). Clone S5/1 has been shown to inhibit the binding of C5a to its receptor. We recommend the use of SM1182A for functional studies.
Formulation	PBS State: Purified State: Liquid purified Ig fraction Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	complement component 5a receptor 1
Database Link	Entrez Gene 728 Human UniProt ID P21730
Background	CD88 is predominantly expressed on cells of the myeloid lineage.
Synonyms	C5a-R, C5aR, C5AR1, Complement Component 5a Receptor 1
Reference Data

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