CD71 (TFRC) Mouse Monoclonal Antibody [Clone ID: DF1513]

Specifications

Product Data
Clone Name	DF1513
Applications	FC, IF
Recommend Dilution	Immunofluorescence. Flow Cytometry:1/10-1/25, Use 10 µl of 1/10-1/25 diluted antibody to label 10e6 cells in 100 µl. Immunohistochemistry on Frozen Tissue Sections only: Use at 10-20 µg/ml with an avidin-biotin system. Western blot: 1-5 µg/ml.
Reactivity	Human, Monkey
Host	Mouse
Clonality	Monoclonal
Immunogen	KG1 cell line. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NSI myeloma cell line.
Specificity	This antibody recognizes the CD71 cell surface antigen, a 190 kD homodimeric glycoprotein expressed by proliferating cells. CD71 acts as Receptor for Transferrin.
Formulation	PBS, pH 7.4 State: Aff - Purified State: Liquid purified IgG fraction Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein G from tissue culture supernatant.
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	90-95 kDa
Gene Name	transferrin receptor
Database Link	Entrez Gene 7037 Human UniProt ID P02786
Background	The transferrin receptor has been structurally characterized as a sulfide bound dimer of identical glycoprotein subunits of 95 kDa. The transferrin receptor is not present on resting blood lymphocytes. On PBL, the receptor appears after activation. The expression of transferrin receptor is coordinately regulated with cell growth. Present on T and B cell lines. The soluble (or serum) transferrin receptor (sTfR) is a circulating truncated form of the membrane receptor protein; it is an 85 kDa glycoprotein forming in serum a 320 kDa complex with diferric transferrin.The most important clinical use of the sTfR determination is in the differential diagnosis between iron deficiency anaemia and the anaemia of chronic disease.
Synonyms	TfR1, p90, Transferrin receptor protein 1
Reference Data

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