ICAM1 Mouse Monoclonal Antibody [Clone ID: 15.2]

Specifications

Product Data
Clone Name	15.2
Applications	FC, IHC
Recommend Dilution	Flow Cytometry: Use 10 µl of 1/50-1/100 dilution to label 10e6 cells in 100 µl. Immunoprecipitation. Immunohistochemistry on Frozen Sections: 1/50-1/100. Recommended Positive Control Tissue: Human Tonsil.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human monocytes. Spleen cells from immunised BALB/c mice were fused with cells of the mouse Sp2/O-Ag14 myeloma cell line.
Specificity	This antibody recognises the CD54 cell surface antigen. Clone 15.2 is reported to block CD54 function. We recommend the use of SM1156A for such studies.
Formulation	PBS, pH 7.4 containing 0.09% Sodium Azide as preservative. State: Purified State: Liquid purified IgG fraction.
Concentration	lot specific
Purification	Affinity Chromatography on Protein G.
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	intercellular adhesion molecule 1
Database Link	Entrez Gene 3383 Human UniProt ID P05362
Background	CD54 is a 90kD glycoprotein also known as ICAM-1. CD54 is expressed by many cells following activation by inflammatory mediators.
Synonyms	ICAM-1
Reference Data

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