ICAM1 Mouse Monoclonal Antibody [Clone ID: 15.2]

Specifications

Product Data
Clone Name	15.2
Applications	FC, IHC, IP
Recommend Dilution	Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 10e6 cells in 100 µl. Immunohistochemistry on Frozen and Paraffin Embedded Sections: 1/50-1/100. Recommended Positive Control tissue: Human Tonsil. Immunoprecipitation.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human monocytes.
Specificity	This antibody recognizes the human CD54 cell surface antigen. Clone 15.2 blocks CD54 function. We recommend the use of SM1156A for such studies.
Formulation	PBS, pH 7.4 with 0.09% Sodium Azide as preservative. State: Purified State: Liquid purified IgG fraction.
Concentration	lot specific
Purification	Affinity Chromatography on Protein G.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	intercellular adhesion molecule 1
Database Link	Entrez Gene 3383 Human UniProt ID P05362
Background	ICAM1 is a 85-110 kDa single chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. It shares considerable amino acid sequence homology with ICAM3 and with ICAM2. ICAM1 is expressed by activated endothelial cells. It is detected on cells of many other lineages (e.g. epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils), with lower levels that increase in inflammation. ICAM1 is also detected in some carcinoma and melanoma cells. Soluble ICAM1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes. It is the receptor for rhinoviruses and malaria.
Synonyms	ICAM-1
Reference Data

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