CD62P (SELP) Mouse Monoclonal Antibody [Clone ID: AK-6]

Specifications

Product Data
Clone Name	AK-6
Applications	FC, IHC, WB
Recommend Dilution	Western blotting. Flow Cytometry: Use 10 µl of 1/10-1/100 diluted antibody to label 106 cells in 100 µl. Immunohistochemistry on Frozen Sections: 1/1000-1/5000. Immunohistochemistry on Paraffin Embedded Sections: 1/500-1/2000. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections but staining may be enhanced using Sodium Citrate buffer pH 6.0. Recommended Positive Control Tissue: Tonsil.
Reactivity	Human, Monkey
Host	Mouse
Clonality	Monoclonal
Immunogen	Human platelet membrane glycoproteins.
Specificity	This antibody recognizes CD62P. It reacts with a 140 kDa antigen expressed on activated platelets and also on endothelial cells both of blood and lymph vessels.
Formulation	PBS, pH 7.4 State: Purified State: Liquid purified IgG fraction from Tissue Culture Supernatant Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	selectin P
Database Link	Entrez Gene 6403 Human UniProt ID P16109
Background	CD62P (P-Selectin) is an adhesion glycoprotein that is expressed on platelets and endothelial cells upon their activation. Interaction between CD62P and its mucin-like ligand PSGL-1 (P-selectin glycoprotein ligand-1) expressed on the microvilli of most leukocytes supports leukocyte rolling along postkapillary venules at the earliest time of inflammation. Both CD62P and PSGL-1 are extended glycoproteins that form homodimers. CD62P dimerization is probably mediated through interactions of the transmembrane domains and stabilizes leukocyte tethering and rolling, probably by increasing rebinding within a bond cluster.
Synonyms	SELP, GMRP, GRMP, PADGEM, GMP-140, LECAM3
Reference Data

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