CD33 Mouse Monoclonal Antibody [Clone ID: WM53]

Specifications

Product Data
Clone Name	WM53
Applications	FC
Recommend Dilution	Flow Cytometry: Use 10µl of Neat-1/10 diluted antibody to label 10e6 cells in 100 µl.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human AML cells. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NSI myeloma cell line.
Specificity	This antibody recognises the CD33 cell surface glycoprotein.
Formulation	PBS, pH 7.4 containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer. Label: APC State: Lyophilized purified IgG fraction. Label: Allophycocyanin
Reconstitution Method	Restore with distilled water.
Purification	Affinity Chromatography on Protein G.
Conjugation	APC
Storage Condition	Prior to and following reconstitution store the antibody undiluted at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Gene Name	CD33 molecule
Database Link	Entrez Gene 945 Human UniProt ID P20138
Background	CD33 is found on granulocyte and macrophage precursors in the bone marrow, but is not on pluripotent stem cells. The protein is also expressed on, and is a useful marker for, peripheral monocytes. It is also useful for distinguishing myelogenous leukaemia cells from lymphoid or erythroid leukaemias. Identification of human Monocytes (bright) and Granulocytes (dim) expressing the 67kDa M.W. surface antigen. CD33 is a 67kD molecule expressed by monocytes, granulocyte precursors, myeloid progenitor cells and myeloid leukaemias.
Synonyms	Siglec-3, gp67
Reference Data

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