CD18 (ITGB2) Rat Monoclonal Antibody [Clone ID: YFC118.3]

Specifications

Product Data
Clone Name	YFC118.3
Applications	FC, IHC, IP
Recommend Dilution	Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 10e6 cells in 100 µl. Immunoprecipitation. Immunohistochemistry on Frozen Sections: 1/1000-1/5000. Recommended Positive Control tissue: Human Tonsil.
Reactivity	Canine, Human
Host	Rat
Clonality	Monoclonal
Immunogen	Human neutrophils. Spleen cells from immunised LOU rats were fused with cells of the rat Y3/Ag.1.2.3 myeloma cell line.
Specificity	This antibody recognises the human CD18 cell surface antigen, the integrin beta2 subunit. This antibody blocks CD18 mediated homotypic adhesion.
Formulation	PBS, pH 7.4 containing 0.09% Sodium Azide preservative. State: Purified State: Liquid purified IgG fraction.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	integrin subunit beta 2
Database Link	Entrez Gene 3689 Human UniProt ID P05107
Background	CD18 is an integrin. Integrins are integral cell surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, beta 2 combines with the alpha L chain to form the integrin LFA1, and combines with the alpha M chain to form the integrin Mac1. Integrins are known to participate in cell adhesion as well as cell surface mediated signalling. CD18 is expressed by most leucocytes. Defects in the CD18 gene result in leukocyte adhesion deficiency.
Synonyms	Integrin beta-2, MFI7
Reference Data

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