CD14 Mouse Monoclonal Antibody [Clone ID: Tük4]

Specifications

Product Data
Clone Name	Tük4
Applications	ELISA, FC, FN, WB
Recommend Dilution	Flow Cytometry: 1/100 - 1/200; use 10 µl of suggested working dilution to label 1x10e6 cells or 100 µl whole blood. ELISA. Western Blot: under reducing conditions. Functional Assays.
Reactivity	Bovine, Canine, Feline, Goat, Human, Mink, Monkey, Porcine, Rabbit, Sheep
Host	Mouse
Clonality	Monoclonal
Specificity	This antibody recognises the CD14 cell surface antigen, expressed strongly by most peripheral blood monocytes and weakly on granulocytes. Clone Tuk4 has been shown to block SDF-induced chemotaxis of U937 cells in a dose-dependent manner.
Formulation	PBS State: Low Endotoxin State: Liquid purified IgG
Concentration	lot specific
Purification	Affinity chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store the antibody at -20°C. Avoid repeated freezing and thawing.
Gene Name	CD14 molecule
Database Link	Entrez Gene 929 Human UniProt ID P08571
Background	CD14 is a single copy gene encoding 2 protein forms: a 50 to 55 kDa glycosylphosphatidylinositol anchored membrane protein (mCD14) and a monocyte or liver derived soluble serum protein (sCD14) that lacks the anchor. Both molecules are critical for lipopolysaccharide (LPS) dependent signal transduction, and sCD14 confers LPS sensitivity to cells lacking mCD14. Increased sCD14 levels are associated with inflammatory infectious diseases and high mortality in gram negative shock. CD14 also appears to be involved in clearance of gram-negative bacteria via its high affinity binding to LPS-LPB complexes.
Synonyms	CD14
Note	Endotoxin Level: less than 0.01 EU/µg
Reference Data

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