CD4 Mouse Monoclonal Antibody [Clone ID: RPA-T4]

Specifications

Product Data
Clone Name	RPA-T4
Applications	FC, IHC
Recommend Dilution	Flow cytometry: 1/50 - 1/100; Use 10µl of the suggested working dilution to label 10e6 cells or 100µl whole blood. Immunohistochemistry on frozen sections: 1/10 - 1/100. Recommended positive control: Human lymphatic tissue.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human PHA blasts. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Specificity	This antibody recognises CD4. Epitope mapping studies have shown that antibodies, produced by clone RPA-T4, recognise an epitope within domain 1, of the extracellular region, of the CD4 molecule. Clone RPA-T4 has been reported to block gp120-CD4 interaction and inhibit syncytium formation. We recommend the use of SM1058LE for this purpose.
Formulation	PBS, pH7.4 containing 0.09% Sodium Azide State: Purified State: Liquid purified IgG
Concentration	lot specific
Purification	Affinity chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	CD4 molecule
Database Link	Entrez Gene 920 Human UniProt ID P01730
Background	CD4 is a 55kD cell surface glycoprotein that is primarily expressed on a subpopulation of T lymphocytes, on peripheral blood monocytes and on tissue macrophages.
Synonyms	T-cell surface antigen T4/Leu-3
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.