CD4 Mouse Monoclonal Antibody [Clone ID: RPA-T4]

Specifications

Product Data
Clone Name	RPA-T4
Applications	FC
Recommend Dilution	Flow Cytometry: Use 10 µl of neat-1/10 diluted antibody to label 106 cells or 100 µl of lysed whole peripheral blood.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human PHA blasts. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NSI myeloma cell line.
Specificity	This antibody recognizes CD4. Epitope mapping studies have shown that antibodies, produced by clone RPA-T4, recognize an epitope within domain 1, of the extracellular region, of the CD4 molecule. Clone RPA-T4 has been reported to block gp120-CD4 interaction and inhibit syncytium formation. We recommend the use of SM1058LE for this purpose.
Formulation	PBS, pH 7.4 Label: FITC State: Liquid purified IgG fraction from tissue culture supernatant Stabilizer: 1% BSA Preservative: 0.09% Sodium Azide Label: Fluorescein Isothiocyanate Isomer 1
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	FITC
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Gene Name	CD4 molecule
Database Link	Entrez Gene 920 Human UniProt ID P01730
Background	CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.
Synonyms	T-cell surface antigen T4/Leu-3
Reference Data

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