MHC Class II RT1B (monomorphic) Mouse Monoclonal Antibody [Clone ID: OX-6]

Specifications

Product Data
Clone Name	OX-6
Applications	FC
Recommend Dilution	Flow Cytometry: Use 10 µl of Neat-1/10 diluted antibody to label 10e6 cells in 100 µl. This product is routinely tested in Flow Cytometry on rat splenocytes.
Reactivity	Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Rat thymocyte membrane glycoprotein. Spleen cells from immunised BALB/C mice were fused with cells fo the mouse NS1 myeloma cell line.
Specificity	This antibody recognises a monomorphic determinant of the I-A antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells. This antibody cross reacts with certain Mouse strains of MHC haplotypes k and s. Analysis of recombinant mouse strains has mapped the OX6 determinant to the H2IA region. (1, 6) This clone does not react with the Rat BDIX strain due to a defect in RT1B expression.(6)
Formulation	PBS, pH 7.4 containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer Label: FITC State: Liquid purified IgG fraction. Label: Fluorescein Isothiocyanate Isomer 1
Concentration	lot specific
Purification	Affinity Chromatography on Protein G.
Conjugation	FITC
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Background	The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In rats, this complex is referred to as the RT1 region. In mice, this complex is referred to as the H2 region.
Synonyms	HLA Class II, MHC Class 2 RT1B
Reference Data

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