MHC Class II RT1Bu Mouse Monoclonal Antibody [Clone ID: OX-3]

Specifications

Product Data
Clone Name	OX-3
Applications	FC
Recommend Dilution	Flow cytometry.
Reactivity	Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Rat thymocyte membrane glycoproteins. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Specificity	This antibody recognises a polymorphic determinant of rat Ia antigen (RT1.Bu) present on Lewis, Wistar and AO strain rats but not BN, DA or PVG/c strains. This antibody is useful for distinguishing Ia positive cells from different rat strains, e.g. for recognising cells of donor origin in bone marrow reconstituted radiation chimaeras. MRC OX-3 cross reacts with mouse strains of MHC haplotypes b and s, and analysis of recombinant mouse strains showed that the determinants mapped to the I-A region. This antibody recognises Ia antigens on B-cells, dendritic cells and certain epithelial cells.
Formulation	PBS, pH7.4 containing 0.09% Sodium Azide and 1% Bovine Serum Albumin Label: PE State: Lyophilized purified IgG Label: R. Phycoerythrin (RPE)
Reconstitution Method	Restore with 1 ml distilled water
Purification	Ion exchange chromatography
Conjugation	PE
Storage Condition	Prior to and following reconstitution store the antibody at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Database Link	UniProt ID Q70RH8
Reference Data

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