MHC Class II RT1Bu Mouse Monoclonal Antibody [Clone ID: OX-3]

CAT#: SM082PS

MHC Class II RT1Bu mouse monoclonal antibody, clone OX-3, Purified

Size: 100 ug 250 ug



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CNY 4,587.00


货期*
4周

规格
    • 100 ug

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Specifications

Product Data
Clone Name OX-3
Applications FC, IHC
Recommend Dilution Flow Cytometry: Use 10 µl of 1/100 diluted antibody to label 10e6 cells in 100 µl.
This product is routinely tested in flow cytometry on Lewis rat splenocytes).
Immunohistochemistry on Frozen and Paraffin Sections:
This product does not require protein digestion pre-treatment of paraffin embedded sections e.g. trypsin or pronase prior to staining. This product does not require antigen retrieval using heat treatment methods prior to staining of paraffin sections.
Reactivity Mouse, Rat
Host Mouse
Clonality Monoclonal
Immunogen Rat thymocyte membrane glycoproteins.
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Specificity This antibody recognises a polymorphic determinant of rat Ia antigen (RT1.Bu) present on Lewis, Wistar and AO strain rats but not BN, DA or PVG/c strains. This antibody is useful for distinguishing Ia positive cells from different rat strains, e.g. for recognising cells of donor origin in bone marrow reconstituted radiation chimaeras.
MRC OX-3 cross reacts with mouse strains of MHC haplotypes b and s, and analysis of recombinant mouse strains showed that the determinants mapped to the I-A region.
This antibody recognises Ia antigens on B-cells, dendritic cells and certain epithelial cells.
Formulation PBS, pH 7.4 containing 0.09% Sodium Azide as preservative.
State: Purified
State: Liquid purified IgG fraction.
Concentration lot specific
Purification Ion Exchange Chromatography.
Conjugation Unconjugated
Storage Condition Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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