Thy1 Mouse Monoclonal Antibody [Clone ID: OX-7]

Specifications

Product Data
Clone Name	OX-7
Applications	FC
Recommend Dilution	Flow Cytometry: Use 10 µl of Neat-1/10 diluted antibody to label 106 cells in 100 µl. This product is routinely tested on Rat thymocytes.
Reactivity	Guinea Pig, Mouse, Rabbit, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Rat Thy1 antigen. Spleen cells of immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Specificity	This antibody recognises CD90, also known as Thy1.1. Since Thy1.1 is a monomorphic determinant in Rat but polymorphic in mice, clone MRC OX-7 reacts with Thy1.1 mice e.g. AKR and FVB mice, but not Thy1.2 mice such as CBA and BALB/c. Clone MRC OX-7 has been demonstrated to promote neurite outgrowths on peripherin-stained sympathetic neurons, using Fluorescence Microscopy (See Jeng, C .J. et al. for details). The Affinity of the Fab' of MRC OX-7 for Rat Thy1 is 3 x 109m-1 and Mouse Thy1.1 is 3 x 108m-1 (See Mason, D.W. & Williams, A.F. for details). This clone has been reported to induce glomerular nephritis in Wistar rats (See Tamura, M. et al. for details).
Formulation	PBS Label: FITC State: Liquid purified IgG fraction. Stabilizer: 1% BSA Preservative: 0.09% Sodium Azide Label: Fluorescein Isothiocyanate Isomer 1 (Excitation / Emission: 525 nm / 490 nm)
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	FITC
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Gene Name	thymus cell antigen 1, theta
Database Link	Entrez Gene 21838 Mouse UniProt ID P01831
Background	Thy1.1 is expressed on a variety of cell types including thymocytes, neuronal cells, stem cells, T lymphocytes (mouse), immature B cells (rat) and connective tissues.
Synonyms	Thy-1, THY1, CDw90
Reference Data

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