Tfrc Rat Monoclonal Antibody [Clone ID: YTA 74.4]

Specifications

Product Data
Clone Name	YTA 74.4
Applications	FC, IHC, IP
Recommend Dilution	Flow cytometry: 1/50 - 1/100; use 10 µl of the suggested working dilution to label 10e6 cells in 100µl. Immunoprecipitation. Immunohistochemistry on frozen sections.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	Mouse Concanavilin A activated spleen cells. Spleen cells from an immunised DA rat were fused with cells of the Y3/Ag1.2.3 rat myeloma cell line.
Specificity	This antibdoy reacts to the CD71 antigen, the transferrin receptor (TFR). Expressed on all dividing cells and can distinguish resting and activated T-cells. Immunoprecipitates a disulphide linked homodimer of 90kD by SDS PAGE. The antibody inhibits cell proliferation and the mixed lymphocyte response in vitro. Blocks the binding of R17 217.1.3. and R17 208.2 anti-TFR monoclonal antibodies
Formulation	State: Purified State: Liquid purified IgG containing 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	transferrin receptor
Database Link	Entrez Gene 22042 Mouse UniProt ID Q62351
Background	The transferrin receptor has been structurally characterized as a sulfide bound dimer of identical glycoprotein subunits of 95 kDa. The transferrin receptor is not present on resting blood lymphocytes. On PBL, the receptor appears after activation. The expression of transferrin receptor is coordinately regulated with cell growth. Present on T and B cell lines. The soluble (or serum) transferrin receptor (sTfR) is a circulating truncated form of the membrane receptor protein; it is an 85 kDa glycoprotein forming in serum a 320 kDa complex with diferric transferrin. The most important clinical use of the sTfR determination is in the differential diagnosis between iron deficiency anaemia and the anaemia of chronic disease.
Synonyms	TfR1, p90, Transferrin receptor protein 1
Reference Data

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