CD45 / LCA Rat Monoclonal Antibody [Clone ID: YW 62.3]

Specifications

Product Data
Clone Name	YW 62.3
Applications	FC
Recommend Dilution	Flow Cytometry: Use 10 µl of neat antibody to label 10e6 cells in 100 µl. The Fc region of monoclonal antibodies may bind non-specifically to c ells expressing low affinity fc receptors.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	Mouse spleen cells. Spleen cells from an immunised DA rat were fused with cells of the rat Y3/Ag1.2.3 myeloma cell line.
Specificity	This antibody recognises the CD45 cell surface antigen, a 180-220KD glycoprotein, expressed by all leucocytes.
Formulation	PBS, pH 7.4 containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer. Label: PE State: Lyophilized purified IgG fraction. Label: R. Phycoerythrin (RPE)
Reconstitution Method	Restore with 1 ml distilled water.
Purification	Affinity chromatography on Protein G.
Conjugation	PE
Storage Condition	Store the antibody undiluted Prior to and after reconstitution at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Gene Name	protein tyrosine phosphatase, receptor type, C
Database Link	Entrez Gene 19264 Mouse UniProt ID P06800
Background	CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.
Synonyms	PTPRC, Leukocyte common antigen, L-CA, T200
Reference Data

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