Cd40 Rat Monoclonal Antibody [Clone ID: 3/23]

Specifications

Product Data
Clone Name	3/23
Applications	ELISA, FC, FN, IHC
Recommend Dilution	Flow Cytometry: Use 10 µl of 1/50 diluted antibody to label 10e6 cells in 100 µl. Immunohistochemistry on Frozen Sections. ELISA. Functional Assays.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	Extracellular domain of Mouse CD40 and the Fc portion of Human IgG1. Spleen cells from immunised LOU/c rats were fused with cells of the Ag8 mouse myeloma cell line.
Specificity	Specificity was checked by ELISA and flow cytometry using BHK cells transfected with mouse CD40. It does not react with normal mouse Ig or with human IgG1 and will stain most mature mouse B cells. It does not cross react with mouse T cells. Clone 3/23 is a powerful activator of normal B cells especially in the presence of IL-4.
Formulation	PBS, pH 7.4 without preservatives. State: Low Endotoxin State: Liquid purified IgG fraction.
Concentration	lot specific
Purification	Affinity Chromatography on Protein G.
Conjugation	Unconjugated
Storage Condition	Store the antibody at -20°C only. Avoid repeated freezing and thawing.
Gene Name	CD40 antigen
Database Link	Entrez Gene 21939 Mouse UniProt ID P27512
Background	CD40 is a costimulatory molecule of the TNF receptor superfamily and is expressed on many cell types, such as B cells, monocytes/macrophages, dendritic cells, endothelial cells, fibroblasts or vascular smooth muscle cells. Interaction of CD40 and its ligand CD154 (CD40L) is required for the generation of antibody responses to T-dependent antigens as well as for the development of germinal centers and memory B cells. In monocytes/macrophages CD40 engagement induces production of pro-inflammatory cytokines and chemokines. CD40-CD154 interactions are also critical for development of CD4 T cell-dependent effector functions. CD40 links innate and adaptive immune responses to bacterial stimuli and serves as an important regulator affecting functions of other costimulatory molecules.
Synonyms	TNFRSF5, CDw40, Bp50
Note	Endotoxin Level: less than 0.01 EU/µg
Reference Data

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