ATG7 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	ELISA (1/300,000-1/400,000). Western blot (1/500-1/2,000). Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 78 kDa in size corresponding to human Apg7 by western blotting in the appropriate cell lysate or extract. Human Apg7 has been reported to form a homodimer at approximately 160 kDa.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 558-569 of Human Apg7 protein.
Specificity	This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. Reactivity occurs against human Apg7 protein. However, BLAST analysis indicates 100% homology (12/12) for this protein from human, chimpanzee, mouse, rat, dog, and chicken sources. The core amino acids of the immunogen, G-D-S-T-R-D-R-T are 100% identical (8/8) in D. melanogaster. Reactivity with Apg7 proteins from other sources is not known.
Formulation	0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide as preservative State: Purified State: Liquid (sterile filtered) purified IgG fraction Stabilizer: None
Concentration	lot specific
Purification	Immunoaffinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	autophagy related 7
Database Link	Entrez Gene 10533 Human UniProt ID O95352
Background	Apg7 is the human gene product similar in homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae Apg7. In the yeast, the protein appears to be required for fusion of peroxisomal and vaculuolar membranes. The protein shows homlogy to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes.
Synonyms	APG7-like, Autophagy-related protein 7
Note	Protein Sequence: Human Apg7, 703 aa, predicted MW 78.0 kDa 1 maaatgdpgl sklqfapfss aldvgfwhel tqkklneyrl deapkdikgy yyngdsaglp 61 arltlefsaf dmsaptparc cpaigtlynt ntlesfktad kkllleqaan eiwesiksgt 121 alenpvllnk fllltfadlk kyhfyywfcy palclpeslp liqgpvgldq rfslkqieal 181 ecaydnlcqt egvtalpyfl ikydenmvlv sllkhysdff qgqrtkitig vydpcnlaqy 241 pgwplrnflv laahrwsssf qsvevvcfrd rtmqgardva hsiifevklp emafspdcpk 301 avgweknqkg gmgprmvnls ecmdpkrlae ssvdlnlklm cwrlvptldl dkvvsvkcll 361 lgagtlgcnv artlmgwgvr hitfvdnaki sysnpvrqpl yefedclggg kpkalaaadr 421 lqkifpgvna rgfnmsipmp ghpvnfssvt leqarrdveq leqlieshdv vfllmdtres 481 rwlpaviaas krklvinaal gfdtfvvmrh glkkpkqqga gdlcpnhpva sadllgsslf 541 anipgyklgc yfcndvvapg dstrdrtldq qctvsrpgla viagalavel mvsvlqhpeg 601 gyaiasssdd rmnepptslg lvphqirgfl srfdnvlpvs lafdkctacs skvldqyere 661 gfnflakvfn sshsfledlt gltllhqetq aaeiwdmsdd eti
Reference Data

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