Alanine aminotransferase 1 / ALT1 Sheep Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IP, WB
Recommend Dilution	Immunoblotting, Immunoprecipitation and most immunological methods requiring high titer and specificity. ELISA (assayed against 1.0 µg of Alanine Transaminase (ALT) [Pig Heart] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Sheep IgG [H&L] (Goat) and (ABTS (2,2'-azino-bis-[3- thylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested).
Reactivity	Porcine
Host	Sheep
Clonality	Polyclonal
Immunogen	Alanine Transaminase (ALT) [Pig Heart]
Specificity	This antibody detects Alanine Transaminase from heart. Cross reactivity against ALT from other tissues may occur but have not been specifically determined.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 as buffer, 0.01% (w/v) Sodium Azide as preservative State: Serum State: Lyophilized serum
Reconstitution Method	2.0 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Delipidation and defibrination
Conjugation	Unconjugated
Storage Condition	Store vial at 4° C prior to restoration. Restore with deionized water or equivalent. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Database Link	UniProt ID P13191
Synonyms	GPT, AAT1, GPT1
Reference Data

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