DDB2 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IP, WB
Recommend Dilution	Western blot (1/500-1/1,000 - a 47.8 kDa band corresponding to human DDB2 is detected. Most cell lines expressing DDB2 can be used as a positive control). ELISA (1/2,000-1/10,000). Immunoprecipitation (The antibody immunoprecipitates in vitro translated protein and protein from cell lysates (using 293T, and others). Coimmunoprecipitation of related proteins has not been tested.
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide corresponding to amino acids 419-427 of Human DDB2 (C-terminal) coupled to KLH
Specificity	This antibody reacts with DDB2.
Formulation	State: Serum State: Liquid (sterile filtered) monospecific antiserum containing 0.01% sodium azide
Concentration	lot specific
Purification	Processed by delipidation and defibrination followed by sterile filtration
Conjugation	Unconjugated
Storage Condition	Store vial at -20° C prior to opening. This product is stable for one month at 2-8°C as an undiluted liquid. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
Gene Name	damage specific DNA binding protein 2
Database Link	Entrez Gene 1643 Human UniProt ID Q92466
Background	The DDB2 gene encodes the small subunit (p48) of DNA damage-binding protein, which is a heterodimer, composed of a large (p127 DDB1) and a small subunit. The DDB2 subunit appears to be required for DNA binding. This nuclear protein functions in nucleotide-excision repair resulting from UV-damaged DNA by binding to pyrimidine dimers. Its defective activity causes the repair defect in the patients with xeroderma pigmentosum complementation group E (XPE). XP-E is a rare human autosomal recessive disease characterized by solar sensitivity, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities. However, it remains for mutation analysis to demonstrate whether the defect in XPE patients is in this gene or the gene encoding the large subunit.
Synonyms	DNA damage-binding protein 2, UV-DDB2, DDB p48 subunit, DDBb
Reference Data

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