GFP Mouse Monoclonal Antibody [Clone ID: 9F9.F9]

Specifications

Product Data
Clone Name	9F9.F9
Applications	ELISA, IHC, WB
Recommend Dilution	Designed to detect GFP and its variants in ELISA (sandwich or capture), Immunoblotting and Immunoprecipitation. Monoclonal and polyclonal forms of anti-GFP assayed by ELISA for direct binding of antigen recognize wild type, recombinant and enhanced forms of GFP. Monoclonal and polyclonal forms anti-GFP assayed in a sandwich ELISA are well suited to titrate GFP in solution using either form of the antibody as the capture or detection antibodies. The detection antibody is typically conjugated to biotin and complexed with streptavidin-HRP (Cat.-No RA021HRP). Fluorochrome conjugated anti-GFP was assayed by Immunofluorescence microscopy on prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and was shown to detect GFP containing inserts. Significant amplification of signal was detected using fluorochrome conjugated anti-GFP relative to the fluorescence of GFP alone. Peroxidase conjugated anti-GFP assayed by Immunoblot shows a 42 kDa band when reacted with GFP on a Western blot. ELISA: 1/20,000-1/80,000. Western blot: 1/2,000-1/5,000. Immunohistochemistry: 1/500-1/2,500.
Reactivity	A. victoria
Host	Mouse
Clonality	Monoclonal
Immunogen	The immunogen is a GST-Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria
Specificity	Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Peroxidase and purified and partially purified Green Fluorescent Protein (Aequorea victoria) Serum. Reactivity is observed against recombinant Green Fluorescent Protein (recombinant GFP from Aequorea victoria) by both Western blot and ELISA. No reaction is seen against RFP.
Formulation	0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 Label: HRP State: Lyophilized purified Ig fraction from tissue culture supernatant Stabilizer: 10 mg/ml BSA (IgG and Protease free) Preservative: 0.01% (w/v) Thimerisol Label: Horseradish Peroxidase
Reconstitution Method	Restore with 1.0 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	HRP
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Database Link	UniProt ID P42212
Background	Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology. YFP differs from GFP due to a mutation at T203Y; antibodies raised against full-length GFP should also detect YFP and other variants.
Synonyms	Green fluorescent protein, GFP-Tag
Reference Data

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