Ferret IgA, IgG, IgM (H+L chain) Goat Polyclonal Antibody

Specifications

Product Data
Recommend Dilution	Suitable for Immunoblotting (WB 1/2,000-1/10,000) ELISA ( 1/20,000-1/100,000), Immunohistochemistry (1/1000-1/5000), Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. Recommended Dilutions: This product has been assayed against 1.0 ug of Ferret IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/1,000 to 1/4,000 is suggested for this product.
Reactivity	Ferret
Host	Goat
Clonality	Polyclonal
Immunogen	Ferret IgG, IgA and IgM whole molecules.
Specificity	This product was prepared from polyspecific antiserum by immunoaffinity hromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-Goat Serum. This product is suitable for the detection of all Ferret immunoglobulin classes, isotypes and chain combinations.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) sodium azide as preservative. Label: Biotin State: Lyophilized purified Ig fraction. Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Molar radio: 10-20 BAC molecules per Goat IgG molecule.
Reconstitution Method	Restore with 1.0 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Immunoaffinity chromatography.
Conjugation	Biotin
Storage Condition	Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid repeated freezing and thawing.
Synonyms	Ferret IgA, Ferret IgG, Ferret IgM
Reference Data

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