Ferret IgM (mu chain specific) Goat Polyclonal Antibody

Specifications

Product Data
Recommend Dilution	Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: This product has been assayed against 1.0 ug of Ferret IgM in a standard capture ELISA using pNPP (p-nitrophenyl phosphate) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:2,500 of the reconstitution concentration is suggested for this product.
Reactivity	Ferret
Host	Goat
Clonality	Polyclonal
Immunogen	Ferret IgM µ chain
Specificity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Ferret IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Ferret IgM and Ferret Serum. Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse or human heavy or light chain isotypes.
Formulation	0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 with 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) sodium azide as preservative. Label: AP State: Liquid (sterile filtered) purified Ig fraction. Label: Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
Concentration	lot specific
Purification	Immunoaffinity chromatography.
Conjugation	AP
Storage Condition	Store the antibody undiluted at 2-8°C. DO NOT FREEZE! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Dilute only prior to immediate use.
Synonyms	Ferret Immunoglobulin M
Reference Data

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