CREB1 (N-term) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, EMSA, IP, WB
Recommend Dilution	Western Blot: 1/5,00-1/1,000. This antibody was assayed against a lysate of 1 x 106 Raji B cells by Immunoblot and found to be reactive against CREB-1 (p43), showing a 46 kDa band at a dilution of 1/500-1/1,000 followed by reaction with Peroxidase conjugated anti-Rabbit IgG (H&L) [Goat] (Cat#R1364HRP) ELISA: 1/5,000-1/25,000. Immunoprecipitation. Gel Shift: This antibody was also tested in a Gel Supershift Assay and found to be reactive against CREB-1 (p43) using 0.5-1.0 µl per assay.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	CREB-1 (p43) peptide corresponding to a region near the N-terminus of the Human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Specificity	This product was prepared from monospecific antiserum by delipidation and Immunoadsorption against an E.coli lysate immobilized on agarose beads. This antibody may react non-specifically with other proteins. A partial cross-reactivity is observed against CREM-1 protein.
Formulation	0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 State: Liquid State: Liquid (sterile filtered) Serum Stabilizer: None Preservative: 0.01% (w/v) Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody (in aliquots) at -20°C. Dilute only prior to immediate use. Avoid repeated freezing and thawing.
Gene Name	cAMP responsive element binding protein 1
Database Link	Entrez Gene 1385 Human UniProt ID P16220
Background	Cyclic AMP Response Element Binding protein (CREB) is a basic / leucine zipper transcription factor that binds the cyclic AMP response element (CRE) and activates transcription in response to a variety of extracellular signals including neurotransmitters, hormones, membrane depolarization, and growth and neurotrophic factors. Activation of CREB is dependent upon the phosphorylation of serine 133. Phosphorylation occurs via p44 / 42 MAP kinase and p90RSK and also via p38 MAP kinase and MSK 1. Although CREB will bind DNA independent of its phosphorylation state, only the phosphorylated form is competent as a transcription factor. CREB binding protein (CBP), a transcriptional coactivator that directly interacts with CREB, binds to CREB in the region of serine 133. CREB appears to play an important role in learning and memory. CREB knock out mice show diminished learning ability.
Synonyms	CREB-1
Reference Data

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