ASK1 (MAP3K5) pSer83 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	This phospho specific polyclonal antibody reacts human pS83 ASK1 and shows minimal reactivity by Western blot, ELISA and competitive ELISA with non-phosphorylated ASK1. Although not tested, this antibody is likely functional in RIA, Immunohistochemistry and Immunoprecipitation. Recommended Dilutions: For immunoblotting a 1/1,000 dilution is recommended. A 155 kDa band corresponding to human ASK-1 is detected. Whole cell lysates from SW1353 can be used as a positive control. For ELISA a 1/5,000-1/10,000 dilution is recommended.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	This purified antibody was prepared from rabbit serum after repeated immunizations with a KLH conjugated peptide corresponding to amino acids 76-87 of human ASK-1 protein.
Specificity	This product is an IgG fraction antibody purified from antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. No reaction was observed with ASK-1 from mouse sources. Reactivity with the kinase from other sources has not been determined.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% sodium azide as preservative. State: Purified State: Liquid (sterile filtered) purified Ig fraction.
Concentration	lot specific
Purification	Multi-step process.
Conjugation	Unconjugated
Storage Condition	Store the antibody (undiluted) at 2-8°C for one month or (in aliquots) at -20°C for longer. Dilute only prior to immediate use. Avoid repeated freezing and thawing.
Gene Name	mitogen-activated protein kinase kinase kinase 5
Database Link	Entrez Gene 4217 Human UniProt ID Q99683
Background	ASK-1 (apoptosis signal-regulating kinase 1- also referred to as MEK Kinase-5 or MAPKKK5) is a novel serine/threonine MAP kinase kinase kinase (MAPKKK) component of the mitogen -activated protein (MAP) cascade that is activated in response to extracellular stimuli by cytokines, growth factors and environmental stresses and other factors. Overexpression of ASK-1 induces apoptotic cell death. ASK-1 is expressed in a variety of human and mouse tissues. The overall amino acid sequence identity between the mouse and human ASK1 is 91.9%. ASK-1 interacts with CDKN1A (also known as p21, WAF1, CIP1). Please refer to the reference list at the end of this document for further information.
Synonyms	MAPK/ERK kinase kinase 5, MAPKKK5, MAP3K5
Reference Data

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