wecC Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IP, WB
Recommend Dilution	Western blot: 1/500-1/5,000 Immunoprecipitation: 1/100. ELISA: 1/5,000-1/20,000. This product has been assayed against 1.0 µg of N-Acylmannosamine-1-Dehydrogenase [Pseudomonas] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/4,000 to 1/16,000 of the reconstitution concentration is suggested.
Reactivity	Escherichia coli
Host	Goat
Clonality	Polyclonal
Immunogen	N-Acyl Mannosamine-1-dehydrogenase [E.coli expressed Pseudomonas recombinant sequence].
Specificity	This antibody detects Pseudomonas N-Acylmannosamine-1-dehydrogenase. Cross reactivity against N-Acylmannosamine-1-dehydrogenase from other sources is unknown. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-goat serum as well as purified and partially purified N-Acylmannosamine-1-dehydrogenase [Pseudomonas].
Formulation	0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2 Label: HRP State: Purified State: Lyophilized purified Ig fraction Stabilizer: 10 mg/m BSA (immunoglobulin and protease free) Preservative: 0.01% (w/v) Gentamicin sulfate (Do NOT add Sodium azide!) Label: Horseradish peroxidase
Reconstitution Method	Restore with 0.1 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation	HRP
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Database Link	UniProt ID P27829
Background	N acylmannosamine 1 dehydrogenase belongs to the short chain dehydrogenases/ reductases family (SDR). It acts on acetyl D mannosamine and glycolyl D mannosamine.
Synonyms	wecC, rffD, b3787, JW5599
Reference Data

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