Maltose Phosphorylase Goat Polyclonal Antibody
CAT#: R1151BS
Maltose Phosphorylase goat polyclonal antibody, Biotin, Purified
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CNY 7,688.00
货期*
4周
规格
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Specifications
Product Data | |
Applications | ELISA, IP, WB |
Recommend Dilution | Western blot: 1/500-1/5,000. Immunoprecipitation: 1/100. ELISA: 1/5,000-1/20,000. This antibody has been assayed against 1.0 µg of Maltose Phosphorylase in a standard capture ELISA using peroxidase conjugated streptavidin and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/4,000 to 1/20,000 of the reconstitution concentration is suggested. |
Reactivity | Escherichia coli |
Host | Goat |
Clonality | Polyclonal |
Immunogen | Maltose Phosphorylase from E.coli |
Specificity | This antibody detects Maltose Phosphorylase [E.coli]. Cross reactivity against Maltose Phosphorylase from other sources is unknown. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-goat serum as well as purified and partially purified Maltose Phosphorylase [E.coli]. |
Formulation | 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2 Label: Biotin State: Purified State: Lyophilized purified Ig fraction Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free) Preservative: 0.01% (w/v) Sodium azide |
Reconstitution Method | Restore with 0.1 ml of deionized water (or equivalent). |
Concentration | lot specific |
Purification | Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer |
Conjugation | Biotin |
Storage Condition | Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. |
Background | Maltose phosphorylase is a dimeric enzyme that catalyzes the conversion of maltose and inorganic phosphate into beta D glucose 1 phosphate and glucose without requiring any cofactors, such as pyridoxal phosphate. The enzyme is part of operons that are involved in maltose/malto oligosaccharide metabolism. |
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