ABC3735 Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IP, WB
Recommend Dilution	ELISA: 1/1000-1/2500. Immunoprecipitation: 1/100. Western blot: 1/500-1/1000.
Reactivity	Bacillus sp.
Host	Goat
Clonality	Polyclonal
Immunogen	Uricase from Bacillus species
Specificity	This product detects Uricase (Bacillus species). Cross reactivity against uricase from other sources is unknown.
Formulation	0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2 Label: HRP State: Purified State: Lyophilized purified Ig fraction Stabilizer: 10 mg/mL BSA - immunoglobulin and protease free Preservative: 0.01% Gentamicin sulfate. (Do NOT add Sodium azide!)
Reconstitution Method	Restore with 0.1 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation	HRP
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Dilute only prior to immediate use. Avoid repeated freezing and thawing.
Database Link	UniProt ID Q5WBJ3
Background	Uricase is the peroxisomal enzyme responsible for the degradation of urate into allantoin. Uricase is a protein of 300 to 400 amino acids, its sequence is well conserved. It is mainly localised in the liver. In legumes, 2 forms of uricase are found: in the roots, the tetrameric form; and, in the uninfected cells of root nodules, a monomeric form, which plays an important role in nitrogen-fixation.
Synonyms	URIC, URO, UOX, EC 1.7.3.3, Urate oxidase
Reference Data

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