Urease Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IP, WB
Recommend Dilution	Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and most immunological methods requiring high titer and specificity. Recommended Dilutions: ELISA: 1/5,000-1/20,000. Western Blot: 1/500-1/5,000. Immunoprecipitation: 1/100. Note: This product has been assayed against 1.0 µg of Urease [Jack Bean] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/1,000 to 1/3,000 of the reconstitution concentration is suggested for this product.
Reactivity	Jack Bean
Host	Rabbit
Clonality	Polyclonal
Immunogen	Urease [Jack Bean].
Specificity	Assay by Immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Urease [Jack Bean]. Cross reactivity against Urease from other tissues and species may occur but have not been specifically determined.
Formulation	0.01M Sodium Phosphate, 0.14M Sodium Chloride, pH 7.4 State: Serum State: Lyophilized Antiserum Stabilizer: None Preservative: 0.01% Sodium Azide
Reconstitution Method	Restore with 2.0 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Prepared from monospecific antiserum by a Delipidation and Defibrination
Conjugation	Unconjugated
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Database Link	UniProt ID P07374
Background	Urease is a protein that is commonly secreted from the bacterium H. Pylori and is integral to the immune response within the gastric system. Urease carries out a key enzymatic reaction in converting urea into free ammonia and carbonic acid. Urease is commonly derived from the Jack Bean plant in scientific research as the amino acid sequences are highly conserved between species and it is an abundant source of the protein. Anti-Urease Antibody is ideal for investigators in Immunology, Enzymology, and Cell Biology research.
Synonyms	Urea amidohydrolase
Reference Data

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