PKM (pan m-PK) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IP, WB
Recommend Dilution	Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation. Recommended Dilutions: This product has been assayed against 1.0 µg of Pyruvate Kinase [Rabbit Muscle] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:12,000 of the reconstitution concentration is suggested for this product.
Reactivity	Rabbit
Host	Goat
Clonality	Polyclonal
Immunogen	Pyruvate Kinase from Rabbit Muscle.
Specificity	Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Pyruvate Kinase [Rabbit Muscle]. Cross reactivity may occur against other mammalian Pyruvate Kinase, but were not specifically determined.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide. State: Serum State: Lyophilized purified Ig fraction
Reconstitution Method	Restore with 2.0 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Prepared from monospecific antiserum by delipidation and defibrination.
Conjugation	Unconjugated
Storage Condition	Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid repeated freezing and thawing.
Database Link	Entrez Gene 100008676 Rabbit UniProt ID P11974
Synonyms	PK2, PK3, PKM, CTHBP, M2-PK, THBP1, OIP3, OIP-3, Pyruvate kinase 2/3, Pyruvate kinase M1/M2, Pyruvate kinase muscle
Reference Data

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