OVAL Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, IP, WB
Recommend Dilution	ELISA: 1/5,000-1/25,000. Western blot: 1/1,000-1/5,000. Immunohistochemistry: 1/500-1/2,500. Immunoprecipitation: 1/100.
Reactivity	Chicken
Host	Rabbit
Clonality	Polyclonal
Immunogen	Native Ovalbumin from hen egg white
Specificity	This antibody detects Chicken (Gallus gallus) Ovalbumin. Cross reactivity against Ovalbumin from other tissues and species may occur but have not been specifically determined. Immunoelectrophoresis give a single precipitin arc against anti-peroxidase, anti-rabbit serum as well as purified and partially purified Ovalbumin [hen egg white].
Formulation	0.02M Potassium phosphate, 0.15M Sodium chloride, pH 7.2 Label: HRP State: Purified State: Lyophilized purified IgG fraction Stabilizer: 10 mg/ml BSA (immunoglubulin and protease free) Preservative: 0.01% (w/v) Gentamicin sulfate (Do NOT add Sodium azide!) Label: Horseradish peroxidase
Reconstitution Method	Restore with 0.1 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation	HRP
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	ovalbumin (SERPINB14)
Database Link	Entrez Gene 396058 Chicken UniProt ID P01012
Background	Chicken Ovalbumin is the major protein in the "white" of the egg (and a favorite antigen in immunological research). Egg white contains a variety of proteins including ovalbumin, conalbumin, ovomucoid and lysozyme. It belongs to the serpin family and the Ov serpin subfamily. Ovalbumin can cause an allergic reaction in humans. Ovalbumin has been implicated in the development of the egg shell. Immunohistochemistry revealed that ovalbumin is found only in the mammillary bodies of decalcified shell, and is not distributed throughout the shell matrix. These results indicate that ovalbumin is present during the initial phase of shell formation and becomes incorporated into the protein matrix of the mammillary bodies. However, it is not yet clear whether ovalbumin at this site plays a specific role in shell mineralisation.
Synonyms	SERPINB14, Egg albumin, Plakalbumin, Allergen Gal d II
Reference Data

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