LDHA Goat Polyclonal Antibody

CAT#: R1094

LDHA goat polyclonal antibody, Serum



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CNY 20,681.00


货期*
4周

规格
    • 2 ml

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Specifications

Product Data
Applications ELISA, IF, IP, WB
Recommend Dilution Suitable for use in ELISA (1/4,000-1/20,000) and Western blot (1/500-1/2,000), Immunoprecipitation (1/100). Expect a band approximately 36 kDa in size corresponding to LDH western blotting in the appropriate cell lysate or extract.
Reactivity Rabbit
Host Goat
Clonality Polyclonal
Immunogen Full length lactate dehydrogenase protein isolated from Rabbit muscle.
Specificity Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Lactate Dehydrogenase [Rabbit Muscle].
BLAST analysis was used to determine that cross reactivity is suggested for both muscle and heart isoforms (LDH-A and LDH-B) from most mammalian species.
Formulation 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2
State: Serum
State: Lyophilized purified Ig fraction
Stabilizer: None
Preservative: 0.01% Sodium Azide
Reconstitution Method Restore with 2.0 ml of deionized water (or equivalent)
Concentration lot specific
Purification Prepared from monospecific antiserum by delipidation and defibrination
Conjugation Unconjugated
Storage Condition

Prior to reconstitution store at 2-8°C.
Following reconstitution store undiluted at 2-8°C for one month
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.

Background Lactate dehydrogenase is also known as L-lactate dehydrogenase A chain, LDH-A, LDH muscle subunit and LDH-M. Two isozymes of LDH occur in mammals, LDH-M and LDH-H which come together to form a homotetramer of 36 kDa subunits. Every LDH molecule consists of four subunits, where each subunit is either H each M (based on their electrophoretic properties.) There are, therefore, five LDH isotypes: LDH-1 (4H) - in the heart, LDH-2 (3H1M) - in the reticuloendothelial system, LDH-3 (2H2M) - in the lungs, LDH-4 (1H3M) - in the kidneys and LDH-5 (4M) - in the liver and striated muscle. Usually LDH-2 is the predominant form in the serum. An LDH-1 level higher than the LDH-2 level (a "flipped pattern") suggests myocardial infarction (damage to heart tissues releases heart LDH, which is rich in LDH-1, into the bloodstream). In general, LDH is often used as a marker of tissue breakdown.
LDH shows a cytoplasmic localization.
Synonyms LDH-A, L-lactate dehydrogenase A chain, LDH-M, PIG19, NY-REN-59
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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