Glucose Oxidase Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IP, WB
Recommend Dilution	Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and most immunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 ug of Glucose Oxidase [Aspergillus niger] in a standard sandwich ELISA using peroxidase conjugated affinity purified anti-rabbit IgG and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/4,000-1/16,000 of the reconstitution concentration is suggested.
Reactivity	Aspergillus
Host	Rabbit
Clonality	Polyclonal
Immunogen	Glucose Oxidase [Aspergillus niger].
Specificity	Immunoelectrophoresis give in a single precipitin arc against purified and partially purified Glucose Oxidase [Aspergillus niger]. Cross reactivity against Glucose Oxidase from other tissues and species may occur but have not been specifically determined.
Formulation	0.01 M Sodium phosphate, 0.15 M Sodium chloride, pH 7.2 containing no preservatives/stabilizers State: Serum State: Lyophilized purified Ig faction.
Reconstitution Method	Restore with 2.0 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Prepared from monospecific antiserum by a delipidation and defibrination.
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Dilute only prior to immediate use. Avoid repeated freezing and thawing.
Database Link	UniProt ID P13006
Background	Glucose Oxidase is widely used for the determination of glucose in body fluids and in removing residual glucose or oxygen from foods and beverages. Glucose Oxidase producing moulds such as Aspergillus and Penicillium species are used for the biological production of gluconic acid. CATALYTIC ACTIVITY: Beta D glucose + O2 = D glucono 1,5 lactone + H2O2.
Synonyms	Glucose oxyhydrase, Beta-D-glucose:oxygen 1-oxido-reductase, gox
Reference Data

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