gdh Goat Polyclonal Antibody

CAT#: R1082HRPS

gdh goat polyclonal antibody, HRP, Purified



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CNY 7,688.00


货期*
4周

规格
    • 100 ug

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Specifications

Product Data
Applications ELISA, WB
Recommend Dilution Western blot: 1/1,000-1/5,000.
ELISA: 1/10,000-1/40,000.
This product has been assayed against 1.0 µg of Glucose dehydrogenase [Bacillus sp.] in a standard captureELISA using  ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/1,000 to 1/3,000 of the reconstitution concentration is suggested for this product.
Reactivity Bacillus sp.
Host Goat
Clonality Polyclonal
Immunogen Glucose dehydrogenase from Bacillus sp.
Specificity This antibody detects Glucose dehydrogenase [Bacillus sp.]. Cross reactivity against Glucose dehydrogenase from other sources is unknown. 
Immunoelectrophoresis give a single precipitin arc against anti-peroxidase, anti-goat serum as well as purified and partially purified Glucose dehydrogenase [Bacillus sp.].

Formulation 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
Label: HRP
State: Purified
State: Lyophilized purified Ig fraction
Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free)
Preservative: 0.01% (w/v) Gentamicin sulfate (Do NOT add Sodium azide!)
Label: Horseradish peroxidase
Reconstitution Method Restore with 0.1 ml of deionized water (or equivalent).
Concentration lot specific
Purification Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation HRP
Storage Condition Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Background Glucose dehydrogenase catalyses the oxidation of D glucose without prior phosphorylation to D beta gluconolactone using NAD or NADP as a coenzyme. The enzyme is a tetrameric protein, each of the 4 identical subunits containing 262 amino acid residues. This family is a subset of a more general family of short chain dehydrogenases and reductases.
Synonyms Glucose 1-dehydrogenase, gdh, BSU03930
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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