Fructose 6 Phosphate Kinase Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation. Recommended Dilutions: This product has been assayed against 1.0 µg of Fructose-6-Phosphate Kinase [Rabbit Muscle] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:6,000 of the reconstitution concentration is suggested for this product.
Reactivity	Rabbit
Host	Goat
Clonality	Polyclonal
Immunogen	Fructose-6-Phosphate Kinase from Rabbit Muscle.
Specificity	Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Fructose-6-Phosphate Kinase [Rabbit Muscle]. Cross reactivity may occur against other mammalian Fructose-6-Phosphate Kinase, but were not specifically determined.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide as preservative. State: Serum State: Lyophilized purified Ig fraction.
Reconstitution Method	Restore with 2.0 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Prepared from monospecific antiserum by delipidation and defibrination.
Conjugation	Unconjugated
Storage Condition	Store vial at 2-8°C prior to restoration. Centrifuge product if not completely clear after standing at room temperature. For extended storage aliquot reconstituted contents and freeze at -20°C or below. Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
Background	Phosphofructokinase catalyzes the irreversible conversion of fructose 6 phosphate to fructose 1,6 bisphosphate. Mammalian PFK is a complex isozyme consisting of 3 subunits: muscle (M), liver (L), and platelet (P). Each subunit is encoded by a separate structural locus on chromosomes 1(M), 21(L), and 10(P). PFKL is the major form in liver and kidney while only M type PFK isozyme is expressed in mature muscle; therefore, muscle contains only homotetramers of M subunits. Erythrocytes contain both L and M subunits, and these randomly tetramerize to form M4, L4, and 3 additional hybrid forms of the holoenzyme.
Reference Data

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