Choline oxidase Goat Polyclonal Antibody
CAT#: R1074HRPS
Choline oxidase goat polyclonal antibody, HRP, Purified
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CNY 7,688.00
货期*
4周
规格
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Specifications
Product Data | |
Applications | ELISA, IP, WB |
Recommend Dilution | Western blot: 1/500-1/1,000. Immunoprecipitation: 1/100. ELISA: 1/1,000-1/2,500. This product has been assayed against 1.0 µg of Choline oxidase [Alcaligenes spec.] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. |
Reactivity | Alcaligenes |
Host | Goat |
Clonality | Polyclonal |
Immunogen | Choline oxidase from Alcaligenes spec. |
Specificity | This product detects Choline oxidase from Alcaligenes spec. Cross reactivity against Choline Oxidase from other sources is unknown. Immunoelectrophoresis give a single precipitin arc against anti-peroxidase, anti-goat serum as well as purified and partially purified Choline Oxidase [Alcaligenes spec.]. |
Formulation | 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2 Label: HRP State: Purified State: Lyophilized purified Ig fraction Stabilizer: 10 mg/m BSA (immunoglobulin and protease free) Preservative: 0.01% (w/v) Gentamicin sulfate (Do NOT add Sodium azide!) Label: Horseradish peroxidase |
Reconstitution Method | Restore with 0.1 ml of deionized water (or equivalent). |
Concentration | lot specific |
Purification | Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer |
Conjugation | HRP |
Storage Condition | Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. |
Database Link | |
Background | Choline oxidase catalyzes the oxidation of choline and betaine aldehyde to betaine with concomitant consumption of oxygen and production of hydrogen peroxide. |
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