Choline oxidase Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IP, WB
Recommend Dilution	Western blot: 1/500-1/1,000. Immunoprecipitation: 1/100. ELISA: 1/1,000-1:2,500. This product has been assayed against 1.0 µg of Choline oxidase [Alcaligenes spec.] in a standard capture ELISA using peroxidase conjugated streptavidin and ABTS as a substrate for 30 minutes at room temperature.
Reactivity	Alcaligenes
Host	Goat
Clonality	Polyclonal
Immunogen	Choline oxidase from Alcaligenes spec.
Specificity	This product detects Choline oxidase from Alcaligenes spec. Cross reactivity against Choline Oxidase from other sources is unknown. Immunoelectrophoresis give a single precipitin arc against anti-biotin, anti-goat serum as well as purified and partially purified Choline Oxidase [Alcaligenes spec.].
Formulation	0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2 Label: Biotin State: Purified State: Lyophilized purified Ig fraction Stabilizer: 10 mg/m BSA (immunoglobulin and protease free) Preservative: 0.01% (w/v) Sodium azide
Reconstitution Method	Restore with 0.1 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation	Biotin
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Database Link	UniProt ID P16101
Background	Choline oxidase catalyzes the oxidation of choline and betaine aldehyde to betaine with concomitant consumption of oxygen and production of hydrogen peroxide.
Reference Data

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