Choline oxidase Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and most immunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 ug of Choline Oxidase [Alcaligenes species] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:32,000 of the reconstitution concentration is suggested for this product.
Reactivity	Alcaligenes
Host	Goat
Clonality	Polyclonal
Immunogen	Choline Oxidase [Alcaligenes species].
Specificity	Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Choline Oxidase [Alcaligenes species]. Cross reactivity against Choline Oxidase from other sources is unknown.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% sodium azide as preservative. State: Serum State: Lyophilized Serum
Reconstitution Method	Restore with 2.0 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Prepared from monospecific antiserum by a delipidation and defibrination.
Conjugation	Unconjugated
Storage Condition	Store vial at 2-8°C prior to restoration. Centrifuge product if not completely clear after standing at room temperature. For extended storage aliquot contents and freeze at -20°C or below. This product is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
Database Link	UniProt ID P16101
Background	Choline Oxidase catalyzes the oxidation of choline and betaine aldehyde to betaine with concomitant consumption of oxygen and production of hydrogen peroxide.
Reference Data

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