CAT Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IP, WB
Recommend Dilution	Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation, Conjugation and most immunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 ug of Catalase [Bovine liver] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) (R1364HRP) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:26,000 to 1:142,000 of the reconstitution concentration is suggested for this product.
Reactivity	Bovine
Host	Rabbit
Clonality	Polyclonal
Immunogen	Catalase from Bovine liver.
Specificity	Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Catalase [Bovine Liver]. Cross reactivity against Catalase from other tissues and species may occur but have not been specifically determined.
Formulation	0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.4 containing no preservative. State: Serum State: Lyophilized Serum
Reconstitution Method	Restore with 5.0 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Prepared from monospecific antiserum by a delipidation and defibrination.
Conjugation	Unconjugated
Storage Condition	Store vial at 2-8°C prior to restoration. Centrifuge product if not completely clear after standing at room temperature. For extended storage aliquot contents and freeze at -20°C or below. This product is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
Database Link	Entrez Gene 531682 Bovine UniProt ID P00432
Background	Catalase is the classical marker for peroxisomes and is the most abundant protein within peroxisomes. It is found in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide.
Synonyms	CAT, Peroxisome Marker
Reference Data

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