lacZ Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, WB
Recommend Dilution	This product is designed for fluorescent Western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Recommended Dilutions: Western blot: 1/10,000. IF microscopy: 1/500-1/2,500.
Reactivity	Escherichia coli
Host	Rabbit
Clonality	Polyclonal
Immunogen	Beta-galactosidase from E.coli
Specificity	Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum as well as purified and partially purified beta galactosidase (E.coli).
Formulation	0.02M Potassium phosphate, 0.15M Sodium chloride, pH 7.2 Label: FITC State: Purified State: Lyophilized purified IgG fraction Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free) Preservative: 0.01% (w/v) Sodium azide Label: Fluorescein isothiocyanate (molecular weight 390 daltons) Absorption emission: 495 nm / 528 nm Molar radio: 3.5 moles FITC per mole of IgG
Reconstitution Method	Restore with 0.1 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation	FITC
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Database Link	UniProt ID P00722
Synonyms	Beta-Gal tag, lacZ tag, b0344, JW0335, Beta-Gal Fusion Protein, Lactase
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.