ansB Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IP, WB
Recommend Dilution	Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunohistochemistry, Immunoprecipitation and most immunological methods requiring high titer and specificity. Recommended Dilutions ELISA: 1/20,000-1/100,000. Western Blot: 1/2,000-1/10,000.
Reactivity	Escherichia coli
Host	Rabbit
Clonality	Polyclonal
Immunogen	Asparaginase from E. coli
Specificity	Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Asparaginase [E. coli]. Cross reactivity against Asparaginase from other tissues and species may occur but have not been specifically determined.
Formulation	0.02M Potassium phosphate, 0.15M Sodium Chloride, pH 7.2 State: Serum State: Lyophilized antiserum Stabilizer: None Preservative: None
Reconstitution Method	Restore with 2.0 ml of deionized water or equivalent.
Concentration	lot specific
Purification	Prepared from monospecific antiserum by delipidation and defibrination
Conjugation	Unconjugated
Storage Condition	Store vial at 2-8°C prior to restoration. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid. For extended storage aliquot contents and freeze at -20°C or below. Avoid repeated freezing and thawing. Dilute only prior to immediate use.
Database Link	UniProt ID P00805
Background	Asparaginase is an enzyme purified from E. coli and Erwinia carotovora. It acts by deaminating extracellular L asparagine, an amino acid that appears to be essential for protein synthesis by some tumour cells which are unable to synthesise asparagine. Asparaginase from Erwinia carotovora is serologically and biochemically distinct from asparaginase from E. coli, although its antineoplastic activity and toxicity is similar. Asparaginase is usually considered to be cell cycle phase nonspecific, but it may block some cells in G1 or S phase. Asparaginase reduces cellular and humoral immunity. E.coli contains two L asparaginase isoenzymes: L asparaginase I, a low affinity enzyme located in the cytoplasm, and L asparaginase II, a high affinity secreted enzyme.
Synonyms	ansA, L-asparaginase I, L-ASNase I
Reference Data

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