phoA Rabbit Polyclonal Antibody
CNY 20,681.00
货期*
4周
规格
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Specifications
Product Data | |
Applications | ELISA, IP, WB |
Recommend Dilution | Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and most Immunological methods requiring high titer and specificity. Note: This product has been assayed against 1.0 ug of Alkaline Phosphatase [E.coli] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) (Cat#R1364HRP) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/20,000 to 1/100,000 of the reconstitution concentration is suggested for this product. Recommended Dilutions: ELISA: 1/8,000-1/40,000 Western Blot: 1/500-1/2,000 |
Reactivity | Escherichia coli |
Host | Rabbit |
Clonality | Polyclonal |
Immunogen | Alkaline Phosphatase [E.coli]. |
Specificity | This product was prepared from monospecific antiserum by a Delipidation and Defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Alkaline Phosphatase [E.coli]. No cross reactivity occurs against the mammalian form of Alkaline Phosphatase. |
Formulation | 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide as preservative State: Serum State: Lyophilized purified Ig fraction |
Reconstitution Method | Restore with 2.0 ml of deionized water (or equivalent). |
Concentration | lot specific |
Purification | Delipidation and Defibrination |
Conjugation | Unconjugated |
Storage Condition | Store vial at 2-8°C prior to restoration. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid. For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below. Dilute only prior to immediate use. Avoid cycles of freezing and thawing. |
Database Link | |
Synonyms | phoA, b0383, JW0374 |
Reference Data |
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