phoA Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IP, WB
Recommend Dilution	Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and most Immunological methods requiring high titer and specificity. Note: This product has been assayed against 1.0 ug of Alkaline Phosphatase [E.coli] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) (Cat#R1364HRP) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/20,000 to 1/100,000 of the reconstitution concentration is suggested for this product. Recommended Dilutions: ELISA: 1/8,000-1/40,000 Western Blot: 1/500-1/2,000
Reactivity	Escherichia coli
Host	Rabbit
Clonality	Polyclonal
Immunogen	Alkaline Phosphatase [E.coli].
Specificity	This product was prepared from monospecific antiserum by a Delipidation and Defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Alkaline Phosphatase [E.coli]. No cross reactivity occurs against the mammalian form of Alkaline Phosphatase.
Formulation	0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide as preservative State: Serum State: Lyophilized purified Ig fraction
Reconstitution Method	Restore with 2.0 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Delipidation and Defibrination
Conjugation	Unconjugated
Storage Condition	Store vial at 2-8°C prior to restoration. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid. For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below. Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
Database Link	UniProt ID P00634
Synonyms	phoA, b0383, JW0374
Reference Data

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